Practical Microbiology for Wastewater Treatment and Operations

Upcoming dates (2)

Sep. 9-11, 2025

Madison, WI or Live Online

Course Overview

(Note that this course was formerly titled "The Role of Microorganisms in Wastewater Treatment")

Wastewater treatment plants depend heavily on the work of microorganisms. This course will help you understand and manage biological processes and improve your wastewater treatment projects and facilities. You will also learn how to use microscopes to monitor and improve wastewater treatment operation, with hands-on practice with microscopes. 

Course topics and features will include:

  • Managing your microbes – and understanding their needs
  • Bacteria, protozoa, metazoa
  • Floc formation
  • Providing the right environment
  • Effects of pH, alkalinity, and toxins
  • Higher life forms
  • Filamentous organisms
  • Microbiology and practical applications for operations and treatment
  • Wastewater Process control testing
  • Microscope Basics
  • Performing microscope exams
  • Smears and Stains: Gram, PHB, and Neisser
  • Microbiology of Biological Nitrogen and Phosphorus Removal
  • Exercises and microscope demonstrations

Who Should Attend?

  • Wastewater engineers, operators and managers
  • Consulting engineers
  • Federal agencies and military bases
  • Wastewater equipment manufacturers
  • Others interested in wastewater treatment
In-person attendees will be able to practice using microscopes during the course while online attendees will be guided through microscope use and sample identification.

Course Outline

  • Review of Wastewater Treatment Concepts, Terminology, and Processes
  • Overview of Wastewater Microbiology and Microorganisms
    • Physiology, growth and reproduction
    • Environmental factors and microbial ecology
    • Bacteria 
      • Shapes, types, sizes
      • Origin and growth
      • Autotrophs, heterotrophs
      • Aerobic, anaerobic, facultative bacteria
  • Importance of Higher Life Forms
    • Protozoa, Rotifers, etc
      • Populations in wastewater treatment
      • Environmental factors
      • Control strategies
  • Microscope Basics
  • Performing Microscope exams
  • Process Control Testing
  • Filamentous Organisms
    • Viscous bulking, scum formation
    • Effects on activated sludge floc and settleability
    • Control strategies 
  • Creating Smears, Staining, etc
  • Basic microbiology of Biological Nitrogen and Phosphorus Removal
  • Troubleshooting, Case Studies, Examples
  • Microscopic observation (throughout course)
    • Throughout the course, class exercises, discussions, and microscope demonstrations will be included, to help attendees learn and retain key points.

Instructors

Steven Leach

Steven Leach is a senior staff scientist with Novozymes Biologicals, Inc. He has more than 30 years of experience with wastewater biology, including fermentation, filament identification, treatment process evaluation, and onsite analysis during upsets, chlorination, and recovery. He has worked on the installation and start-up of over 20 fixed-film reactors units throughout the country. Steve is widely recognized for his expertise in identifying filaments and protists. He has completed onsite visits to wastewater treatment plants throughout North and South America, Europe, and Asia.

Toni Glymph-Martin

Toni is a Water & Wastewater Microbiologist and Consultant with Wastewater Microbiology Solutions providing technical assistance, consulting, and microscopic evaluations for more than 30 Industries and 80 municipalities.  She advises facility planning and plan review engineers in operational trends and problems to ensure that treatment plant design approvals are consistent with policy and include necessary process control components for optimizing conditions for microbiological treatment. 

Toni served as a senior level supervisor in the Monitoring and Research Division of the Metropolitan Water Reclamation District of Greater Chicago (District). For more than 10 years her responsibilities included, the development of wastewater microbiology related protocols and procedures, including bacteriology, virology, parasitology, and molecular microbiology. Prior to that Toni served as Department expert in Wastewater Treatment Microbiology at the Department of Natural Resources in Wisconsin which included developing written technical guidance documents and providing technical assistance and on-site assistance to treatment plant operators and consultants. 

She has conducted over 500 technical workshops, and has made numerous presentations at conferences, public meetings, at the Great Lakes Commission meetings, and for State Governments. Toni is also a certified operator and has considerable knowledge of the principles, practices and procedures of environmental science as it relates to water quality monitoring and regulations; water and wastewater treatment; and environmental research. She has more than 45 years of water quality related experience.

Adib Amini

Adib Amini, Ph.D., PE, ENV SP, BCEE serves as Program Director in the College of Engineering’s Office of Interdisciplinary Professional Programs, with a focus on Water, Wastewater, Stormwater, and Sustainability. Dr. Amini also works with cities and industries as an engineering consultant, with experience in both drinking water and wastewater treatment. Dr. Amini has expertise in sustainability, engineering design, technology innovation, and renewable energy. Dr. Amini’s work has included the design of novel technologies, including on-site energy systems at facilities. He spearheaded the designs for the first wastewater treatment facilities in Iowa that are 100% powered by on-site renewable energy systems. Dr. Amini invented a novel system for ventilation of rated spaces, such as wastewater headworks buildings, for which he holds a provisional patent. Dr. Amini also has performed award-winning research in technology innovation and sustainability. Dr. Amini has spoken widely at conferences on topics related to water/wastewater treatment, sustainability, PFAS, on-site energy systems, and resilient infrastructure.

Upcoming dates (2)

Program Director

Adib Amini

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